Binding studies of SV40 T-antigen to SV40 binding site II

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Binding studies of SV40 T-antigen to SV40 binding site II.

SV40 T-Antigen binding site II was synthesized, cloned and analyzed for its ability to bind purified SV40 T-antigen. We report the binding constant of T-antigen for isolated site II. Using a filter binding assay the calculated binding constant was 6-8 fold less efficient than site I previously reported. Binding constants were calculated using two methods. The first was a direct calculation usin...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1985

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/13.18.6621